ISSN:
0006-3592
Keywords:
fluorescence confocal microscopy
;
microfabrication
;
aminosilane
;
mercaptosilane
;
antibody immobilization
;
heterobifunctional crosslinker
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Fluorescence confocal microscopy was used to characterize micron-sized microfabricated silicon particles and planar oxide surfaces after silanization and immobilization of IgG antibody. Surfaces treated with amino- and mercaptosilanes were tested for the presence of amine and sulfhydryl groups by labeling with specific fluorescein probes. In addition, human antibody (IgG) was immobilized to the thiol-coated microparticles using the heterobifunctional crosslinker succinimidyl 4-(N-maleimidolmethyl)-cyclohexane-1-carboxylate. Estimates of the surface density of IgG were consistent with 8.3% of a monolayer of covalently-bound antibody. Confocal images confirmed uniform layers of both silanes and antibodies on the microparticles. The sensitivity limit for the confocal measurements was determined to be as low as 1.5 × 10-5 fluors per nm2. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 137-146, 1998.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
