ISSN:
0006-3592
Keywords:
enzymes
;
glycosidases
;
transglycosylations
;
supersaturated solutions
;
glycosides
;
disaccharides
;
synthesis
;
regioselectivity
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Enzymatic transglycosylation in supersaturated solutions of substrates was investigated using crude glycosidase preparations from barley, snail, and coffee beans. It was shown that the use of supersaturated glycoside solutions as media for transglycosylation reactions offers considerable advantages over conventional aqueous systems. These advantages include higher yields, more efficient use of the donor glycosides and improved volumetric productivity, especially in the case of poorly water-soluble substrates. The regioselectivity of the glycosylation was not significantly affected by high concentrations of acceptor glycosides. It was also shown that the regioselectivity of transfer could be directed towards secondary hydroxyl groups by the use of methyl 6-O-acetyl-α-galactopyranoside as acceptor. The value of these approaches was demonstrated by the synthesis of methyl 3- and 4-O-β-D-galactopyranosyl-α-D-galactopyranosides and methyl 3-O-β-D-galactopyranosyl-α-L-fucopyranoside on a preparative scale. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 197-203, 1998.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
