ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The waste mycelium of Aspergillus niger ‘Z’ used to produce citric acid was recognized as a rich source of intracellular acid phosphatases (E.C. 3.1.3.2.). Three forms of enzyme were isolated and purified 50-150 fold according to a procedure involving ammonium sulphate fractionation, Sephadex G-200 gel chromatography, and DEAE-cellulose ion-exchange chromatography.The cytoplasmic form (80% of the whole activity) was found to have molecular weight 230000, ‘MICHAELIS’ constant 2.4 mM (when measured with pNPP), optimal temperature 60°C, pH optimum 1.8-3.0 and contained 30% of carbohydrate in the molecule. This form was able to hydrolyse pNPP, beta-glycerophosphate, inorganic pyrophosphate, glucose-6-phosphate, phytate, compounds and phosvitin, but did not hydrolyse beta-casein nor leeithin.One of two forms connected with the cell walls shown similar properties, whereas the other had molecular weight of 9500, ‘MICHAELIS’ constant 1.4 mM, pH optimum 5.0 and was much less thermostable.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370100402
