ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
To understand and control the dynamics of microbial growth and metabolism, a theoretical background based on segregated models is necessary, and therefore flow cytometry is the suitable measuring method.It is shown that additional information to the usual mean-value information, and to the well-known cytometric methods for determining DNA content and size/shape measurement by scattered light, like the distribution of membrane sterol content of baker's yeast, leads to a more detailed knowledge of growth dynamics.We have found, that a low content of membrane sterols is a characteristic of cells with suppressed proliferation activity, but necessary for high fermentation activity. Also, a high content of membrane sterols seems to enhance the viability and survival capability of harvested and dryed baker's yeast cells. Therefore, we recommend harvesting cells at a point of medium content of membrane sterols.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370120503
