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Investigations of subtilisin (Novo type) immobilization on cellulose by means of various methods of support activation (1994)

Trzmiel, T. ; Galas, E. ; Fortak, M.

Berlin : Wiley-Blackwell

Acta Biotechnologica 14 (1994), S. 205-209

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ISSN: 0138-4988

Keywords: Life Sciences ; Life Sciences (general)

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: The immobilization of subtilisin, Novo type, on WHATMAN cellulose activated by the glutaraldehyde, diisocyanate, diazo-coupling, and S-triazine method, and on a dialdehyde derivative of cellulose has been studied. The best results of proteinase immobilization were achieved when cellulose was activated by hexamethylene diisocyanate. The preparations of a proteolytic activity of 70 PU/g of support and of an esterase activity of 2.3 EU/g of support were obtained with 14% and 38% yield, respectively.

Additional Material: 1 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/abio.370140215

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