ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Wistar strain albino rats were injected with 1 cm3 of a 1% solution of trypan blue. Three types of dye were used: Matheson, Coleman and Bell, Chroma-Gesellschaft and a highly purified sample. The dyes were injected on days 6, 7, and 8 of gestation. Fetuses were excised from days 16 through 20, fixed, decalcified where necessary, embedded in paraffin, serially sectioned and stained. Newborn young were treated similarly. Chroma-Gesellaschaft trypan blue was without reproductive or teratogenic effect at the above doses. Increasing the dose did not increase the teratogenicity but did yield a decrease in litter size. Both Matheson, Coleman and Bell trypan blue and the purified sample were teratogenic. The most frequent neural defect observed was hydrocephalus. Serial sections of the newborn hydrocephalics showed an occluded or extremely tenotic aqueduct of Sylvius in 31 of 33 sectioned animals. The fetuses collected from days 18 through 20 also had occluded or stenotic aqueducts if hydrocephalic. Aqueductal stenosis or occlusion was present in 17-day-old fetuses, but hydrocephalic was not conclusively demonstrated at this age. Sixteen-day-old fetuses did not have aqueductal occlusion in any of those examined, but stenosis was evident. It is concluded that the defect predisposing to hydrocephalus in the young of trypan blue treated rats of this strain is aqueductal stenosis or occlusion.
Additional Material:
13 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091480407
