ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
The morphology of satellite cells was determined by examining brom formalin fixed tissue stained by the silver carbonate technique, and tissue fixed in gluteraldehyde, post-fixed in osmium and prepared for electron microscopy.Light microscopy indicated that oligodendrocytes and astrocytes were the predominant perineuronal cell types. Cells with intermediate morphological characteristics were also observed. The light microscopic criteria for cellular indentification were the comparative size and shape of the cell bodies, and the number and nature of glial processes.Electron microscopy revealed perineuronal cells with round to oval shaped cell bodies, dense nuclear and cytoplasmic matrices, and a rich compliment of organelles. A second, less numerous, type of satellite cell had a larger, oval, elongate, or somewhat angular cell body, light nuclear and cytoplasmic matrices, and gliofibrils and glycogen. The former correlated well with the light microscopic oligodendrocyte and the latter with the astrocyte. “Dark cells” were not observed in a satellite position with the electron microscope nor were cells observed that conform to Hortega's “spiny microglia” in our silver preparations. Specializations of the plasma membranes of satellite glial cells and neurons were observed, and were interpreted as possible morphological evidence for “transport” between neurons and neuroglia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091610112
