ISSN:
0886-1544
Keywords:
cytoskeleton
;
microtubule-associated protein (MAP)
;
marine egg extracts
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Alkaline pH favors the assembly of microtubules (MTs) in marine egg extracts [Suprenant and Marsh, 1987: J. Cell Sci. 184:167-180; Suprenant, 1989: Exp. Cell Res. 184:167-180; 1991: Cell Motil. Cytoskeleton 19:207-220] and mammalian brain extracts [Tiwari and Suprenant, 1993: Anal. Biochem. 215:96-103], even though the assembly of purified microtubule protein (MTP) from both of these sources is favored at slightly acidic pH. The present investigation examines whether alkaline pH has a direct or indirect effect on MT nucleation and growth in soluble brain extracts. Cell-free extracts were prepared from bovine cerebral cortex, and a nucleated assembly assay was used to demonstrate that MT assembly in brain extracts is favored at slightly acidic pH. The increase in MT mass found at alkaline pH is due to an increase in the solubility of tubulin not an increase in the extent of assembly On average, 47.7 ± 11.3% of the total tubulin is soluble at pH 7.2, while only 30.9 ± 8.9% of the tubulin is soluble at pH 6.8. A model is proposed that indicates how microtubule proteins from both mammalian brain and marine eggs may be associated with pH-dependent factors. © 1994 Wiley-Liss, Inc.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970280107
