ISSN:
0021-8383
Schlagwort(e):
Chemistry
;
Organic Chemistry
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
Spectrophotometric studies of mixtures containing Cr(iii) and bovine serum albumin were carried out to know the binding of chromic ion to the serum protein. Experiments performed at varying metal: protein ratio and at different pH values gave a maximum at 580 mμ. An inflexion was obtained in molar extinction coefficient-pH curves at a pH value corresponding to the pK values of carboxyl groups. All these go to prove that the carboxyl groups are likely to be the Prinzipal sites through which the fixation of chromic ions have been affected. Experiments carried out in the neutral pH region gave no evidence regarding the binding of metal ions to the imidazole groups of bovine serum albumin. The lesser reactivity of bovine serum albumin in comparison with that of transfusion gelatin (previously studied) has been attributed to the lack of effective multipoint sites, due to its folded structure, so as to fix several chromium atom intramicellarly.
Zusätzliches Material:
2 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/prac.19650280304
