ISSN:
0360-6376
Keywords:
Physics
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The interaction between polyelectrolytes and Acridine Orange (AO) has been investigated in the case of poly(acrylic acid) (PAA), poly(methacrylic acid) (PMA), and a polycondensate between 1,3-benzene disulfonyl chloride and L-lysine (PLL) by visible absorption and fluorescence spectroscopy. The influence of both polymer ionization and polymer/dye ratio (P/D) on the spectral behavior of the bound dye has been studied. The stacking tendency of AO is found lower in the presence of PLL under compact conformation (in an ionization range depending on the nature of the counterion) with correlated enhancement of the green fluorescence of the monomeric species of the bound dye. The disappearance of the green fluorescence and the dimerization of bound AO are directly related to the increased flexibility of polyion chains upon ionization. Some analogy is found between the behavior of bound AO in the presence of very compact PLL chains and that reported for AO in the presence of native DNA, which might be due to specific interactions responsible for a kind of “intercalation” of the monomeric bound dye.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/pol.1978.170160108
