ISSN:
1420-9071
Keywords:
Superoxide
;
free radical
;
reactive oxygen species
;
chemiluminescence
;
pancreatitis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer, and lucignin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8±337.7 counts/10 sec) were not different from those of females (335±308.7 counts/10 sec) (p=0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522±2014 counts/10 sec) were significantly higher than those of healthy controls (p〈0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01923359
