ISSN:
1573-6776
Keywords:
alcohol oxidase
;
gene expression
;
human growth hormone
;
Pichia pastoris
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Mature human growth hormone (hGH) cDNA was cloned by homologous recombination into the yeast Pichia pastoris genome. The hGH gene expression was placed under the control of the methanol-inducible alcohol oxidase 1 (AOX1) gene promoter and the Saccharomyces cerevisiae α-factor signal sequence to direct the secretion of recombinant human growth hormone (rhGH) into the growth medium. O2-limited induction of recombinant yeast strains in shake tubes with 3 ml of culture medium produced up to 11 mg rhGH l−1, while high cell density cultures using a 2-l bioreactor produced about 49 mg rhGH l−1 achieving 40% of total protein of the culture medium supernatant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005675920451
