ISSN:
1570-7458
Keywords:
Aleyrodidae
;
predation
;
biological control
;
serology
;
ELISA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have developed an enzyme-linked immunosorbent assay (ELISA) for detection of whitefly antigen in the guts of predaceous arthropods based on a monoclonal antibody (MAb) produced in response toBemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) egg antigen. We observed positive antigen-antibody reactions with the egg and the adult female stages of certain whitefly species and not with others. This MAb did not cross-react with any of the whitefly nymphs or adult males we tested. Moreover, this MAb did not cross-react with the various life stages of other insect species we examined. This immunologically-based technique to identify whitefly egg facilitates examinations of predator gut contents in the field. Such a specific and sensitive whitefly egg immunoassay will expedite the characterization of theB. tabaci predator complex in the southwestern United States.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02380775
