ISSN:
1573-0603
Keywords:
adipocyte
;
differentiation
;
defined medium
;
triglyceride
;
G3PDH
;
fat pad
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary This paper describes a method to establish primary and secondary cultures of adipocyte precursors isolated from inguinal fat pads of 48-hr-old rats in defined medium. The culture medium consists of DME-F12 medium supplemented with fibronectin, insulin, transferrin, and fibroblast growth factor. Data presented indicate that 90% of the cells plated in the 4F medium differentiate in 7 d. These cultures provide appropriate differentiation assay systems to characterize regulators of differentiation acting on normal cells derived from the adipose tissue.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01404597