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Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases (1976)

Meyerink, J. H. ; Retèl, J. ; Planta, R. J. ; [et al.]

Springer

Molecular biology reports 2 (1976), S. 393-400

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ISSN: 1573-4978

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcription units. They are superimposed on a rather broad smear of background DNA, representing the heterogenous ‘spacer’ sequences. From the restriction maps, together with data obtained by partial digestion, a physical map for the ribosomal transcription unit in yeast could be constructed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00366261

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