ISSN:
1573-0832
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Summary 1. Thirty-two strains ofHistoplasma capsulatum were studied concerning their ability to form tuberculate spores and their conversion into the yeast phase. 2. Nine strains did not produce tuberculate spores on Sabouraud's agar, on corn meal agar, on spent medium, on media with pH adjusted from 4.5 to 7.0 or on the first passage through hamsters. 3. Tuberculate spore production did occur in these nine strains when Sabouraud's medium was enriched with phosphate, especially KH2PO4. In addition, all but two strains produced tuberculate spores after a second passage through hamsters. 4. Growth on KH2PO4 enriched Sabouraud's agar led to a greater yield of yeast phase as compared to yeast phase resulting from colonies of the same strain grown on plain Sabouraud's agar. This may be due to the greater number of spores produced on the KH2PO4 enriched medium. 5. A grinding technique of preparing inocula improved slightly the facility of obtaining the yeast phase over heavy inoculation with unground pieces of mold culture.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02053252
