ISSN:
1423-0127
Keywords:
Pseudomonas exotoxin A
;
Vaccination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Pseudomonas exotoxin A (PE) is one of the most potent cytotoxic agents produced byPseudomonas aeruginosa. In this study, we examined the possibility of using PE with a deletion of 38 carboxyl-terminal amino acid residues, designated PE(Δ576–613), for active immunization against PE-mediated disease. We first examined the toxic effects of PE and PE(Δ576–613) on 5- and 9-week-old ICR mice. The results show that the subcutaneous administration of PE(Δ576–613) at a dose of 250 µg was still nontoxic to 5- and 9-week-old ICR mice, while native PE was lethal at a dose of 0.5 and 1 µg, respectively. PE(Δ576–613) was then used to immunize ICR mice. The minimum dose of PE(Δ576–613) that could effectively induce anti-PE antibodies in 5- and 9-week-old ICR mice was found to be 250 ng. However, immunization with 250 ng PE(Δ576–613) failed to protect the immunized mice from a lethal dose of PE. The effective immunization dose of PE(Δ576–613) that could protect mice against a 2 µg PE challenge was found to be 15 µg. In addition, sera obtained from PE(Δ576–613)-immunized ICR mice were able to neutralize PE intoxication and effectively protect mice from PE. Thus, PE(Δ576–613) may be used as an alternative route to new PE vaccine development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02253525
