ISSN:
1573-4943
Schlagwort(e):
DABS-amino acid analysis
;
high-performance liquid chromatography
;
apolipoprotein B-100
;
tryptic peptides
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Abstract A reliable high-performance liquid chromatography (HPLC) method is described for the separation of dimethylamino-azobenzenesulfonyl-amino acid (DABS-AA). The separation is accomplished by reversed-phase chromatography on a Hypersil ODS column (4.6×150 mm) withe a Hewlett-Packard liquid chromatography system. In addition to the developed sample and solvent preparation procedure, this precolumn modification method using dimethylaminoazobenzene sulfonyl chloride (DABS-CL) for amino acid analysis is proved reliable and sensitive. Five apolipoprotein B-100 tryptic peptides, two of them containing cysteine, were demonstrated as examples for the general application of this method in amino acid analysis. It is a useful method for analysis of cysteine- and cysteine-containing peptide and, furthermore, for determination of sulfhydryl and disulfide linkages of proteins.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01024908
