ISSN:
1432-2242
Keywords:
Key words Somaclonal variation
;
Cytoplasmic male sterility
;
Triticale
;
Regeneration
;
Mitochondrial atp6 gene region
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Comparative hybridization analyses of total DNA from fertile and cytoplasmic male-sterile (CMS) triticale plants which had been regenerated from embryogenic callus cultures revealed the organization and variation of the mitochondrial atp6 gene region. In order to compare different developmental phases, we analysed mitochondrial DNA (mtDNA) from both the shoots and full-grown regenerants. Somaclonal variants were identified on the basis of differences in the mtDNA from fertile and CMS triticale. Several shoots as well as all of the full-grown plants analysed showed somaclonal variation. This phenomenon could be traced back to having primarily orginated from the influence of the nuclear background, which give rise to a stoichiometric increase in a rye-specific orf25 gene copy, and a tissue culture-induced combination of fertile and CMS-specific mtDNA organization of the atp6 gene area. The latter event is probably caused by the homologous recombination of repetitive sequences that may be accompanied by selective amplifications.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050288
