ISSN:
1432-0428
Keywords:
Insulin receptor
;
ontogenesis
;
photoreactive labelling
;
tyrosine kinase
;
anti-receptor antibodies
;
rabbit
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The domain structure of the insulin receptor was investigated in liver and brown adipose tissue of developing rabbits. The structure of the binding domain (α-subunit) was analysed after covalent labelling with a 125I photo-reactive insulin analogue. The structure of the tyrosine kinase domain (β-subunit) and the transmission of the hormonal signal from the α-to the β-subunit were analysed by stimulating with insulin the autophosphorylation of the β-subunit. Finally, the immunoreactivity of the receptor in developing tissues was assessed with anti-receptor antibodies. The results show that a functional insulin receptor can be detected at the early stages of fetal development in both tissues and is conserved throughout ontogenesis to adulthood.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00283146
