ISSN:
1432-0428
Keywords:
Pancreatic islets
;
obese-hyperglycemic mice
;
perifusion
;
fluorescence
;
oxidized flavoproteins
;
D-glucose
;
L-leucine
;
(±)-b-BCH
;
α-ketoisocaproic acid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In perifused pancreatic islets, the fluorescence of oxidized flavoproteins (FAD) was recorded continuously. Elevation of glucose concentration in the medium from 0 or 5 mM to 20 mM led to decrease in FAD-fluorescence beginning 10 sec after change of medium. L-leucine (10 mM), (±)-b-BCH (20 mM) andα-ketoisocaproic acid (10 mM) caused typical kinetics of FAD-fluorescence decrease. The results are interpreted to indicate rapid changes of the functional state of B-cell mitochondria induced by the above-mentioned stimulators of insulin release.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01222108
