ISSN:
1432-072X
Keywords:
Spirochaeta species
;
Thermophile ecology and physiology
;
Obligately anaerobic spirochete
;
Polysaccharolytic
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An obligately anaerobic, extremely thermophilic Spirochaeta species was isolated from a thermal spring on the edge of Green Lake on Raoul Island of the Kermadec archipelago. The strain, designated RI 19.B1 (=DSM 6192) displayed the morphological characteristics typical of Spirochaeta species: regularly coiled long thin cells consisting of a crenulated outer sheath surrounding a central coiled protoplasmic cylinder. Between the outer sheath and the protoplasmic cylinder were two longitudinal periplasmic fibrils in a 1-2-1 arrangement, each anchored by an insertion disc near one pole, whereas the other end was not anchored. The strain displayed a strictly anaerobic, chemoorganotrophic, fermentative metabolism and was able to grow on a variety of mono-, di- and polysaccharides, including cellulose. Sugar alcohols, organic and amino acids were not utilized. Growth supplements were not required, but CO2 was required to produce consistent growth. Strain RI 19.B1 had temperature, pH and salinity optima of 64–66°C, pH 6.95 and 0.4% NaCl respectively. The maximum growth temperature and salinity were 73°C and 2.5% respectively. Glucose was fermented to lactate, acetate, carbon dioxide, and hydrogen. Succinate, ethanol and formate were not detected. The strain displayed the resistance to rifampicin typical of Spirochaeta species. The mol % G+C of DNA from strain RI 19.B1 was 52%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00243461
