ISSN:
1432-1106
Keywords:
Spinal cord grafts
;
Intraocular transplantation
;
Spinal cord development
;
Extracellular recording
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Fetal spinal cord tissue was grafted to the anterior chamber of the eye of adult recipients. Transverse segments from the cervical and high thoracic levels were divided in halves which were grafted directly or further divided into ventral horn and dorsal horn parts before grafting. Survival and intraocular growth was monitored through the cornea. Grafts from E14 to E16 grew to final sizes several times the initial size. The final size of E17 grafts was approximately similar to the initial size, while the final size of E18 and E19 grafts was considerably smaller than the size at grafting. All grafts were well vascularized from the host iris. Grafts from younger donors contained several neurons typical of spinal cord including alpha-motoneuron-type cells. Cells were found in clusters in gray matter areas surrounded by white matter. Extracellular recording revealed many spontaneously active cells. Several had high sustained discharge (10–25 Hz) and large amplitudes. Many cells could be excited by stimulation of the graft surface via activation of local afferents. It is concluded that the capacity of fetal spinal cord tissue to survive grafting to the eye chamber is inversely related to the donor age. Before E17, large grafts retaining several morphological and electrophysiological characteristics of spinal cord are obtained. The intraocular spinal cord graft provides a useful model for studies of spinal cord development and, using co-grafting techniques, a model for spinal cord regeneration and functional connectivity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00237016
