ISSN:
1432-1017
Keywords:
Turnip Yellow Mosaic Virus
;
Degradation
;
RNA-release
;
Neutron scattering
;
Hydrostatic pressure
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract The thermal stability of an isometric plant virus, Turnip Yellow Mosaic Virus (TYMV), has been investigated at low and high hydrostatic pressure, using small angle neutron scattering. Contrast variation allowed us to separately observe the structural changes of the protein capsid and the RNA core. The experiments were performed in 0.05M Tris buffer at pD = 8.0 and in 0.05M bis-Tris buffer at pD = 6.0 containing different H2O/D2O mixtures (40% and 70% D2O). It was found that hydrostatic pressure enhances the stability of TYMV. The thermally induced uncoating of RNA as well as structural transitions of the protein capsid are shifted to higher temperature upon increasing the pressure from 5 × 106 Pa to 2 × 108 Pa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01561137
