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Control of glucose-fed batch cultivations of E. coli by means of an oxygen stabilized enzyme electrode (1983)

Cleland, Neil ; Enfors, Sven-Olof

Springer

Applied microbiology and biotechnology 18 (1983), S. 141-147

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary The design of an autoclavable oxygen stabilized electrode based on immobilized glucose oxidase and catalase, with electrolytic generation of oxygen, is presented. The electrode's behaviour was investigated in relation to parameters such as ion strength, buffer capacity, DOT in the broth and enzyme inhibition by Cl−. It was used in fed-batch cultivations of Escherichia coli for control and monitoring of glucose feeding. Use of liquid feeding data from glucose- as well as from pH control for growth characterization is outlined.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00498035

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