ISSN:
1432-0983
Schlagwort(e):
Saccharomyces cerevisiae mitochondria
;
oxi1 gene
;
Maturase-related ORF
;
Transcription initiation
;
End-of-Messenger signal
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary Transcription of the yeast mitochondrial oxi1 gene (cytochrome oxidase subunit 2) is initiated at a variant non a nucleotide sequence, TTAAAAGTA, located 54 by upstream from the protein-coding gene. Transcriptional initiation at this site gives rise to a 2,500 nucleotide primary transcript containing both the oxi1 gene and the downstream maturase-related reading frame, RF1. Precise transcript mapping has revealed that the 3′-end of the mature oxi1 mRNA is generated by an endonucleolytic cleavage which takes place after the conserved dodecamer sequence, AAUAAUAUUCUU (End-of-Messenger signal), 75 nucleotides downstream from the oxi1 stop codon. Since the RF1 5′-terminal coding region overlaps the oxi1 3′-terminal coding sequence, cleavage at this motif truncates the RF1 message suggesting that the expression of the putative RF1 protein is controlled at the level of dodecamer processing.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00387768
