ISSN:
1432-0533
Keywords:
Non-radioactive in situ hybridization
;
Proteolipid protein
;
Myelin basic protein
;
Myelin-associated glycoprotein
;
2′3′-Cyclic nucleotide 3′-phosphodiesterase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A method for in situ hybridization of digoxigenin-labeled cDNA and cRNA probes to myelin protein mRNA is described. This technique has dual advantages of high structural resolution and high sensitivity and avoids problems associated with handling of radioactive materials. Furthermore, it can be readily combined in double labeling with immunocytochemical protein detection. We have used this technique to detect and locate mRNA for myelin basic protein (MBP), proteolipid protein (PLP), 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and myelin-associated glycoprotein (MAG) in oligodendrocytes of 7-day-old and adult rat brains. PLP and MAG mRNA were restricted to the perinuclear cytoplasm, whereas MBP and CNPase mRNA was additionally present in peripheral oligodendrocyte processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00227734
