ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Puparium formation in Drosophila lebanonensis casteeli is obviously restricted to a certain phase in circadian oscillation. The question whether or not the release of molting hormone is the actual process which is controlled by the circadian oscillation could be approached by using molting hormone-specific changes in genome activity as indication for changes in hormone titer. The identification of hormone specific changes in the puffing pattern of polytene chromosomes should provide a basis for this study.—To this end, a chromosome map of the 7 polytene chromosome arms (1 acrocentric and 3 metacentric chromosomes) of the species was made. Changes in the puffing pattern associated with puparium formation are described and compared with those occurring in response to experimental administration of β-ecdysone.—89 puffs were regularly observed in midthird instar larvae. Prior to puparium formation 5 new puffs arise, one at an early stage and 4 attaining their maximum size immediately before puparium formation. Concomitantly, 5 puffs increase considerably in size. These changes in the puffing pattern can be reproduced by injection of ecdysone.—Upon injection of the hormone a clear differentiation between fast reacting loci (within 30–60 min) and slow reacting loci (after 3–4 hours) can be found. As in other Drosophila species the immediate response (within 30–60 min) comprises more than one (5) locus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00284949
