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Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae (1984)

Hirsch, P. R. ; Beggs, J. D.

Springer

Molecular genetics and genomics 195 (1984), S. 209-214

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ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A section of the octopine Ti plasmid pTiACH5 including the region always integrated in crown gall tumour cells (the TL-DNA) was cloned into the yeast — E. coli shuttle vector pJDB207. This plasmid, pOTY8, was transformed into S. cerevisiae where transcription of the T-DNA was demonstrated, but there was no evidence for the production of octopine synthetase. The TL-DNA was cloned into a derivative of pJDB207 unable to replicate in yeast, but the TL-DNA did not appear to contain any autonomously replicating sequences (ars) functional in yeast.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00332748

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