ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The nucleotide sequence of the gene rpsD for the ribosomal protein S4 of three thermosensitive mutants of Escherichia coli K 12 was determined. It was found that two of them contained regional multiplications of a nucleotide sequence within the gene rpsD. In one case, it is a duplication of a 31 nucleotide stretch and in another it is a triplication of a 41 nucleotide stretch. The thermosensitive phenotype of the two mutants is unstable and reverts at the frequency of approximately 10-4. The revertants regain the wild-type nucleotide sequence. We postulate that the two mutant genes that contain regional multiplications possibly take an intra-strands secondary structure, which is cleaved to regenerate the wild-type sequence, probably during DNA replication.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330268