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Digitale Medien

Transferase-deficiency galactosemia: Immunochemical studies of the Duarte and Los Angeles variants (1984)

Andersen, M. W. ; Williams, V. P. ; Sparkes, Maryellen C. ; [weitere]

Springer

Human genetics 〈Berlin〉 65 (1984), S. 287-290

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Details

ISSN: 1432-1203

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Rabbit antibodies to purified human placental galactose-l-phosphate uridyltransferase (EC 2.7.7.12) were used to establish immunologic cross-reactivity patterns for the enzyme in hemolysates, prepared from red cells of a normal individual, a homozygous Duarte variant, and a heterozygous Los Angeles variant. The antibody immunoprecipitated all three forms of the enzyme. The amount of antibody absorbed by each hemolysate was related to the different levels of activity, and examination of hemolysate/antibody reaction mixtures by starch gel electrophoresis revealed that the antibody quantitatively precipitated all of the isoenzyme forms that characterize these three genetic variants.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00286519

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