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Immunoprecipitation of a male-specific polypeptide after in vitro translation of testicular poly(A)+ RNA (1988)

Lattermann, Ulrich ; Müller, Ulrich

Springer

Human genetics 〈Berlin〉 79 (1988), S. 305-308

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ISSN: 1432-1203

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Poly(A)+ RNAs from male and female gonads of 20-day-old rats were translated in vitro using rabbit reticulocyte lysates. The resulting polypeptides were incubated with specific anti-male (anti-H-Y) antisera raisedin female rats by intrasplenic immunization with syngeneic male skin. Using a second antibody, a male-specific polypeptide of molecular weight approximately 18,000 was immunoprecipitated. This male-specific polypeptide was not cotranslationally modified in vitro and appeared to be identical to serological H-Y antigen.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00282166

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