ISSN:
1439-1104
Keywords:
cell culture
;
molluscan neuron
;
hemolymph
;
synaptogenesis
;
neuronal outgrowth
;
H. pomatia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Hemolymph of adultAplysia californica significantly affects neurite outgrowth of identified neurons of the land snailHelix pomatia. The metacerebral giant cell (MGC) and the motoneuron C3 from the cerebral ganglion and the neuron B2 from the buccal ganglion ofH. pomatia were isolated by enzymatic and mechanical dissociation and plated onto poly-l-lysine-coated dishes either containing culture medium conditioned byHelix ganglia, or pre-treated withAplysia hemolymph. To determine the extent of neuronal growth we measured the neurite elongation and the neuritic field of cultured neurons at different time points.Aplysia hemolymph enhances the extent and rate of linear outgrowth and the branching domain ofHelix neurons. With the hemolymph treatment the MGC neuron more consistently forms specific chemical synapses with its follower cell B2, and these connections are more effective than those established in the presence of the conditioned medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02336659
