ISSN:
1432-1424
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Centrifugation techniques are described which allow that glass microelectrodes can be inserted separately into cytoplasm and vacuoles of intact cells of the mossMnium cuspidatum. This provides unequivocal measurements of the plasmalemma potential (E co between cytoplasm and outer medium). For various reasons, which are discussed in detail, determinations of the tonoplast potential (E vc between vacuole and cytoplasm) remain ambiguous. In the moss cells equilibrated with artificial pond water (APW),E co is highly negative (i.e. −130 to −190 mV).E vc is either close to 0 mV or positive (+60 to +65 mV) depending on the interpretation of the measurements. However, there is no reason to suggest that under these conditions highly negative potentials occur at the tonoplast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01870119
