ISSN:
1437-773X
Schlagwort(e):
Key words Helicobacter pylori
;
Amoxicillin
;
New methods for EM preparation
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract A new method for preparing electron microscopic specimens of Helicobacter pylori was developed and used to examine the ultrastructure of this bacterium. We have also investigated the morphological changes in the bacterium when exposed to amoxicillin using our new method. Bacterial specimens for electron microscopy are usually prepared by collecting the bacteria by centrifugation during the fixation and dehydration processes. In our new method the bacteria are filtered through and adsorbed onto a filter before fixation, and the entire filter containing the adhered bacteria is fixed and dehydrated. Using this method we were able to obtain electron photomicrographs in which the external appearances or internal structures of the bacteria were well conserved. The advantages of this method are that it uses only a small amount of bacterial suspension, shortens the time required for the dehydration procedure, and keeps the artifacts to the minimum. Amoxicillin treatment resulted in coccoid form with blebs in the bacterial surface and the appearance of vacuoles, granules, and an area of low electron density in the cytoplasm at one and four minimum inhibitory concentrations. These changes were consistent with results previously reported in the literature.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s007950050010
