ISSN:
1437-773X
Keywords:
Key words Mitogen-activated protein kinase (MAPK)
;
Ischemia reperfusion injury
;
Heart
;
Ultrastructure
;
Immunohistochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The mitogen-activated protein kinase (MAPK) family is considered to be activated by stress, but the role of the MAPK family is still unknown in cardiac pathology. In the present study, not only the localization of MAPKs such as the extracellular responsive kinase (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK (p38), but also ultrastructural changes were investigated in the ischemia-reperfusion model of Wistar rats. At 5, 10, 30, 60, and 180 min reperfusion after 30 min ischemia by occluding the coronary artery, the expression of these MAPKs was increased in blood vessels and cardiomyocytes by Western blotting and immunohistochemical methods. In addition, after ischemia reperfusion, various ultrastructural changes such as decreased glycogen granules, mitochondrial swelling, and myolysis were observed in the blood vessels and cardiomyocytes. These results suggest that protein kinases may regulate numerous biological processes, including the regulation of contraction and ion transport.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007950070005
