ISSN:
1662-9752
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The molecular weight and crystallinity of systems based on poly(L-lactic acid) PLLA is an important issue as it can influence, besides the general physical properties of the polymer, the patterns of cell adhesion, proliferation and cell morphology. The objective of the present study was to evaluate how crystallinity and molecular weight of PLLA influence the referred parameters. Four conditions were tested: low molecular weight amorphous and semi-crystalline PLLA disks, andhigh molecular weight amorphous and semi-crystalline PLLA disks, obtained from hot press. The thermal properties of the studied materials were accessed by differential scanning calorimetry. For the cell culture studies a human osteosarcoma cell line (SaOS-2) was chosen. Disks were immersed in a cell suspension containing 5x104 cells/ml and kept in culture for periods up to two weeks. Cellviability and proliferation of SaOS-2 cells was assessed by MTS test and a total protein assay, respectively. The adhesion and morphology of SaOS-2 cells on PLLA disks was assessed by scanning electronic microscopy. Results showed that cell viability was not affected by the different tested conditions. However, cell proliferation was increased in the high molecular weight amorphous samples and cells seemed to have higher adhesion patterns on semi-crystalline samples.This is probably happening due to different rates of integrin interaction with the substrate leading to different patterns of focal adhesion points formation
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/02/12/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.514-516.1020.pdf
