ISSN:
1744-313X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Intracytoplasmic μ-positive pre-B cells (μ+VH315− cells) transformed with Abelson virus continuously produced cells (μ+VH315+ cells) that were double-stained by anti-μ and anti-VH315 antibodies which specifically recognized the immunoglobulin heavy chain variable region identical or closely related to that of MOPC315 myeloma protein. Southern blot analysis indicated that the μ+VH315+ cells were generated from the μ+VH315- cells by the VH315·D·JH2 to a germline JH3 joining on the non-productive VH315·D·JH2 allele, which resulted in the production of μ-chains with variable region detectable by anti-VH315 antibodies. Therefore, it is strongly indicated that VHDJH to JH joining is not blocked in μ-chain-producing pre-B cells, and thus is free from allelic exclusion machinery.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1744-313X.1990.tb00891.x
