ISSN:
1744-313X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Biosynthetically labelled Qa-2 antigens were isolated from mouse spleen cells by immunoprecipitation with anti-Qa-2 antisera. When newly synthesized Qa-2 molecules from several different inbred strains were analysed by two-dimensional (2D) gel electrophoresis; four different phenotypes were observed that differed in the number of polypeptides present. The ability to distinguish Qa-2+ phenotypes was used to map the recombination points in two congenic strains, B6. Tlaa and A. Tlab. No alternative Qa-2-like polypeptides were detected in B6.K1 (Qa-2−) cells using a polyspecific rabbit antiserum against mouse class I antigens, but a new molecule was detected in BALB/cBy (Qa-2−) cells. Pulse-chase and surface-labelling experiments showed that some, but not all, of the newly synthesized Qa-2 precursor forms are processed to mature cell surface molecules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1744-313X.1987.tb00366.x
