Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
26 (1976), S. 0
ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Abstract— Four antisera active against choline acetyltransferase (ChAc) were obtained by injecting 22 rabbits with rat brain ChAc. The ChAc preparations used for immunization (specific activity from 015 to 2 μmol/min/mg of protein) were not pure and the antisera produced were not monospecific. The antisera inhibited and precipitated ChAc, but the precipitated enzyme-antibody complexes still retain ChAc activity. One millilitre of the most active serum precipitates 0–5 μmol/min of rat brain ChAc at the equivalence point. Its titre expressed in mg/ml of immunoglobulins precipitated with the antigen and the equivalence point was calculated at about 0.08 mg/ml of serum. This relatively low titre explains the lack of any visible ChAc immunoprecipitate in an immunodiffusion test. Cross-reactivity studies revealed that ChAc has undergone few changes during evolution, since antisera produced against rat brain ChAc still precipitate ChAc from fish (Torpedo).
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1471-4159.1976.tb01510.x
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