ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Radioactive adenine has been frequently used to label cyclic adenosine 3′, 5′-monophosphate (cAMP) in diverse cells and tissues (Kuo and DeRenzo, 1969; Shimizu, Daly and Creveling, 1969, Brooker, 1971). We have previously reported that cAMP from the guinea-pig cerebral cortex is derived from a precursor pool of adenine nucleotides which is more highly labelled than the bulk cellular ATP (Shimizu, Creveling and Daly, 1970a). The present study was carried out to throw additional light on the property of the precursor pool. Levels of cAMP and ATP and specific activities of [14C]cAMP and [14C]ATP were determined simultaneously in cerebral slices which were prelabelled with [14C]adenine and subsequently incubated with veratridine, histamine or adenosine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1973.tb00098.x
