ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— Slices of guinea pig cerebral cortex were incubated in bicarbonate- or trisbuffered media. Influx of 45Ca2 +, 54Mn2 +, 133Ba2+ or 90Sr2+ was determined at intervals following addition of the cardiac glycoside, ouabain, to the medium. After incubation for 30 min in the presence of 45Ca2+ and in the absence of ouabain, about 40 per cent of the slice calcium became labelled. Ouabain (01 I″M) markedly increased 45Ca2 + content, as well as the content of unlabelled Ca2 +. Mg2+ and Na+ levels also rose while K+ content dropped at a more rapid rate. Ouabain also brought about increased uptake of 90Sr2+, 133Ba2 + and 54Mn2 +. Lowering the Na+ content of the media prevented the effects of ouabain and brought about an increase in calcium content of brain slices. Addition of 2,4-dinitrophenol (01 mM) or iodoacetate (1 miu) had slight or moderate effects on 45Ca2+ uptake. The data are most compatible with the presence in cerebral tissue of a sodium-calcium exchange process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1971.tb11969.x
