Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
16 (1969), S. 0
ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— [14C]Leucine was injected intracranially into the brainstem reticular formation at the level of the upper medulla by the stereotaxic method. Subcellular fractions prepared 3 hr after injection showed that the specific activities of leucine-incorporated proteins decreased in the order soluble, microsomal, nuclear and mitochondrial fractions. Specific activities of proteins in the sera were 2·2 per cent of whole homogenate proteins.The results from 27 experiments showed that 66·6 per cent of the mean specific activities of proteins extracted from whole homogenates fell within ·1 s.d. and 100 per cent within ± 2 s.d. (close to a normal distribution). The coefficients of variation were between 40 and 50 per cent for whole homogenates, sera and all subcellular fractions. Reproducibility of results and factors concerned with possible errors in the technique are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1969.tb10362.x
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