ISSN:
1745-4514
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A new process was developed to regulate the papain-induced overtenderization of meat by using oryzacystatin, a cysteine proteinase inhibitor of rice seed origin. Meat cubes (2 × 2 × 2 cm each), aged 3 days, were treated with a 0.1 fold weight of papain solution and incubated at 8°C. The most favorable degree of tenderization resulted when a 2% papain solution was used for 2 h at 8°C. When the incubation was made for 24 h or longer, overtenderization resulted. The incubation of a meat cube with papain for the first 2 h and then with an adequate amount of added oryzacystatin for the subsequent 22 h was effective in regulating overtenderization. It was also confirmed by SDS-polyacrylamide gel electro-phoresis that the use of oryzacystatin under similar incubation conditions regulated the papain-aided hydrolysis of myofibrillar proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1745-4514.1991.tb00160.x
