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deoP1 promoter and operator mutants in Escherichia coli: isolation and characterization (1991)

Dandanel, G. ; Hammer, K.

Oxford, UK : Blackwell Publishing Ltd

Molecular microbiology 5 (1991), S. 0

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ISSN: 1365-2958

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Medicine

Notes: Plasmid DNA containing deoP1, one of the two major promoters of the deo operon, has been mutagenized using hydroxylamine, and promoter down-mutations and operator mutations were selected. The isolated mutants are all located within a 16bp palindromic sequence containing the –10 region of deoP1. The results show that RNA polymerase and DeoR repressor compete for the same DNA target. The deoP1 promoter activity is dependent on a TG motif one base pair upstream of the –10 consensus sequence. The sequence of the deo operator site was further verified by use of a synthetic linker.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb02083.x

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