ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Cultured thymic fragments (CTF) from WAG/CPB(RTU and DA/01a (RT1a rats were prepared in the presence or absence of 2′ deoxyguanosine or cyclosporine A, and subsequently transplanted under the kidney capsule of congenitally athymic and euthymic WAG CPB recipients. The rationale of the culture supplements was that these may affect the disappearance of medullary dendritic cells, with subsequent induction of allotolerance. However, the immunohistology of the CTF showed more RTI class II-positive cells than keratin-positive cells, indicative of the maintenance of dendritic cells. Grafts in athymic animals showed the recovery of the original thymic architecture within 6 weeks utter transplantation. The influx of host-derived lymphocytes was accompanied by an influx of dendritic cells in the deculla-like area and macrophages in the cortex. A similar recovery was observed for syngeneic CTF in euthymic recipients. In addition lymphocytic infiltration was seen in the connective tissue surrounding the epithelial areas. Allogeneic grafts in euthymic animals were rejected within 3 weeks after transplantation. This outcome of the transplanted CTF under different conditions was not affected by the supplementation of the thymic culture before transplantation with 2′ deoxyguanosine or cyclosporine A.We conclude that there is no tolerance induction after transplantation in euthymic allogeneic rats of CTF prepared in the presence of 2′ deoxyguanosine. This conclusion is in contrast to date in the mouse, which may be explained by the maintenance of dendrite cells during culture. A chimaeric state of donor-derived epithelium and host-derived dendritic cells is obtained by transplantation of allografts in arthymic rats.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1992.tb03257.x
