ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The proliferative response of guinea-pig thymocytes to co-mitogenic stimulation with phytohaemuglutinin and the guinea-pig interleukin 1 (IL-1)-like lymphocyte-activating monokine was lost by removing the cells that adhere to a Sephadex G-10 (G-10) column or the cells of low density in a Ficoll-Conray gradient. The diminished response in the G-10 non-adherent thymocyte or high-density thymocyte fraction was restored by the addition of a macrophage-depleted 6–10 adherent thymocyte fraction or a low-density. Ia-positive thymocyte fraction but not by the addition of peritoneal macrophages. These results suggest that the accessory cells which mediate the increased responsiveness of thymocytes to the IL-1-like monokine existed in G-10 adherent cell fractions and the cells with this accessory function were not macrophages The accessory cells were shown to DC of low density, glass-non-adherent, G-10-adherent, Fc receptor-negative, and Ia-positive. These results also suggest that the G-10-non-tidherent and high-density thymocyte subpopulation, which is unresponsive or responds very little to the IL-l-like monokine by itself, acquires responsiveness to the monokine and proliferates by Stimulation with the 1L-1-Iike monokine and lectin in the presence of the accessory cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1988.tb01450.x
