ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Cytomodulin (CM-1) is a synthetic heptapeptide. Human dermal fibroblasts cultured in the presence of CM-1 showed increased expression of transforming growth factor–ß(TGF-ß) and collagen I and decreased expression of MMP-1. TGF-β is involved in numerous vital processes in wound healing, and the rate of wound repair is influenced by its overexpression at the site of injury. To establish the effect of CM-1 in wound healing, we administered several concentrations of CM-1 to incisional wounds and to unwounded skin of collagen-luciferase transgenic mice. Luciferase bioluminescence served as a quantitative reporter of CO1A2 transcription. Imaging was performed to measure the luciferase activity on these mice at different time points after administration of 1, 10 and 100 ug CM-1. The highest amount (100 μg) showed the highest level of collagen expression both in unwounded and wounded skin. In another experiment, we used a scrambled peptide as a control and administered the peptides both topically and by injection. CM-1 was effective in stimulating luciferase expression both by topical and intradermal administration. To determine the effect of CM-1 on wound strength, we measured the tensile strength in incisional wounds of wild type C57B6 mice at 10 d. Histological analysis was also performed to assess the collagen content and organization of the tissue. The data showed a statistically significant increase in tensile strength and collagen content at 10 and 100 μg doses. These results illustrate the utility of CM-1 as a novel, low-molecular weight vulnerary agent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractca.x
