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  • Articles: DFG German National Licenses  (3)
  • Electronic Resource  (3)
  • 1990-1994  (2)
  • 1985-1989  (1)
  • 1900-1904
  • Myocyte  (2)
  • Behavior  (1)
Source
  • Articles: DFG German National Licenses  (3)
Material
  • Electronic Resource  (3)
Years
  • 1990-1994  (2)
  • 1985-1989  (1)
  • 1900-1904
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 45 (1989), S. 215-222 
    ISSN: 1420-9071
    Keywords: Behavior ; learning ; leaf surface ; wax ; insects ; food selection ; secondary compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Direct observations on the feeding behavior of insect herbivores are uncommon, but important. The important aspects of host-plant selection by phytophagous insects that have been revealed by such observations are the role of chemicals in the leaf surface, and learning. There are few detailed reports of behavior at the leaf surface, but these indicate that many, if not all, insects exhibit behavior pattenrs that can be interpreted as an examination of the quality of the surface and acceptance or rejection may follow without further testing. A number of experiments show that chemicals from the leaf surface commonly contribute to the acceptability or otherwise of a plant and in most cases so far the active chemicals are of widespread occurrence, not having a specific association with the host plant. Some experiments show that the association between surface chemicals and plant palatability is learned, but in other cases there is evidence of an innate response. Habituation to deterrent chemicals has been demonstrated in the laboratory, but not in the field. Food aversion learning also occurs and may be important in dietary switching by polyphagous insects.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Myocyte ; Intracellular ions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A method for the manufacture of ion-sensitive micro-electrodes, which can be readily used with small single cells, is described in detail. A glass pipette with a tip size of 1 μm, essentially similar to those used as suction electrodes in whole-cell recording, when silanized and with its tip filled with a suitable ion-sensitive resin, producesan ion-sensitive electrode with fast electrical and chemical response times. These electrodes can be applied to the cell membrane of isolated myocytes and penetration achieved without cell damage, by the application of suction. For the estimation of intracellular ionic activities they can be used in conjunction with a separate conventional KCl-filled micro-electrode or a suction voltage electrode. The technique is illustrated by the measurement of intracellular Na+, Ca2+ and pH. It is possible that these electrodes can also be used to measure local changes in ionic activity in restricted areas.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 422 (1993), S. 325-331 
    ISSN: 1432-2013
    Keywords: Myocyte ; Voltage clamp ; Ionic currents ; Oximes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Exposure of isolated guinea-pig ventricular myocytes to the uncharged oximes 2,3 butanedionemonoxine (BDM) and norPAM (but not by the charged PAM) results in a dose-dependent reduction of the duration of the action potential. The nifedipine-sensitive Ca current is fully inhibited by BDM (IC505.8±0.4 mM) and nor PAM but is little affected by PAM. This inhibition is unaltered by the presence of BAY K 8644 but is antagonized by isoprenaline. The effect of isoprenaline is more pronounced when the solution in the patch pipette contains the non-hydrolysable analogue of adenosine 5′-triphosphate, ATPγS (the IC50 is increased to 44.0±5.2 mM). A hastening of the inactivation of the L-type Ca current persists when either 10 mM 1,2-bis(2-aminophenoxy)-ethane-N, N, N′, N-tetraacetic acid (BAPTA) or 3 mM ATPγS is present in the pipette solution or when BAY K 8644 or isoprenaline are present in the bathing fluid. These results suggest that the inhibition of the Ca current is due to the phosphatase-like activity of the oximes but differs in some respects from previous work where a reduced level of phosphorylation is achieved by the introduction of protein kinase inhibitors or protein phosphatases into the sarcoplasm in guinea-pig myocytes. These differences could be explained if Ca channel availability is regulated by at least two sites of cAMP-dependent phosphorylation with oximes able to rapidly dephosphorylate both sites, while one of these sites is not readily dephosphorylated by the endogenous phosphatases.
    Type of Medium: Electronic Resource
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