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  • Articles: DFG German National Licenses  (2)
  • Electronic Resource  (2)
  • Acidosomes  (1)
  • Aging  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Age-related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats (1996)
    Springer
    Anatomy and embryology 194 (1996), S. 489-500 
    Details
    ISSN: 1432-0568
    Keywords: Temporomandibular joint ; Immunohistochemistry ; Proteoglycan ; Aging ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract There is little information available regarding the morphological and biomolecular characteristics of mandibular condylar cartilage. The purpose of this study was to determine the age-related changes in the morphology and immunolocalization of glycosaminoglycans (GAGs) in mandibular condyles. The mandibular condylar cartilages from 4-, 8-, 16-, 32-, and 64-week-old Wistar male rats were examined to verify the localization of chondroitin-4-sulfate (Ch-4S), chondroitin-6-sulfate (Ch-6S) and keratan sulfate (KS) using an indirect immunofluorescent technique with three monoclonal antibodies for glycosaminoglycans, 2-B-6, 3-B-3 and 5-D-4, respectively. Morphologically, the condylar cartilage was a growth cartilage during growing periods, began to differentiate into articular cartilage from the central area of 16-week-old condyles, and became mature articular cartilage at 32 weeks of age. A regional difference was found in the morphological features and distribution of GAGs between the anterior, central, postero-superior and posterior areas of the condyles at each age. The immunohistochemical localizations of these three glycosaminoglycans showed age-related, morphology-dependent changes, from growth cartilage to articular cartilage-like cartilage. Immunoreactions for all of the antibodies decreased progressively with age in the interterritorial matrix, while the pericellular and territorial matrix in the condylar cartilage of the mandible maintained relatively higher immunoreactivity. In conclusion, age-related and regional differences in the localization of glycosaminoglycans Ch-4S, Ch-6S, and KS were found in the mandibular condyles in rats, and these changes are believed to be related to functional and developmental requirements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185995
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Acidification of the young phagosomes ofParamecium is mediated by proton pumps derived from the acidosomes (1997)
    Springer
    Protoplasma 196 (1997), S. 12-20 
    Details
    ISSN: 1615-6102
    Keywords: Acidosomes ; Concanamycin B ; Phagosomes ; Proton pumps ; Paramecium ; Vacuolar ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although it is generally accepted that phagosome acidification is induced through the activity of a vacuolar proton pump (V-ATPase) present on the phagosome membrane, exactly how these pumps are delivered to the phagosomes is not well understood. To study this question inParamecium, it was necessary to first show that an authentic V-ATPase was present on their phagosomal membranes. Three antibodies raised against V-ATPases or their subunits were each found to label one or two large digestive vacuoles (DVs) inParamecium multimicronucleatum when immunofluorescence microscopy was used. Using horseradish peroxidase immunocytochemistry to increase sensitivity, about 10 DVs were shown to contain a V-ATPase. In high magnification images and cryoultramicrotomy these proton pumps were found to be located on the acidosomes, suggesting the vacuolar proton pumps on the DVs originate from the acidosomes. The authenticity of the V-ATPase was further confirmed by its sensitivity to cold temperature and to the V-ATPase specific inhibitor, concanamycin B, which at 10 nM doubled the t1/2 for vacuole acidification. Thus, we conclude that (1) acidosomes and some DVs ofParamecium have a bona-fide concanamycin B-sensitive and cold-sensitive V-ATPase, (2) the V-ATPase is delivered to the young DVs during acidosome fusion, and (3) the V-ATPase is involved in vacuole acidification. Finally, we have now determined thatParamecium has two immunologically related V-ATPases that are involved in two very different functions, (1) the acidification of phagosomes and (2) fluid segregation in the contractile vacuole complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281054
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    Paper (German National Licenses)
    Fulltext
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